Opakowania handlowe

AmpliTest Helicobacter pylori

(Real Time PCR)


Helicobacter pylori is a gram-negative helical-shaped bacterium containing several (usually up to six) twigs. It lives in conditions of low oxygen availability, mainly on the surface of epithelial cells in the pre-pyloric mucosa of the stomach and duodenum. H. pylori is responsible for approximately 80% of gastric ulcers and 90% of duodenal ulcers. It is estimated that in Poland about 84% of adults and about 32% of people under 18 are infected, but most infected people do not develop the disease. The usual route of infection is still controversial. The oral-oral or faecal-oral route are considered as occurring the most frequently.

AmpliTest Helicobacter pylori (Real Time PCR) test is designed to detect DNA sequences specific for bacteria Helicobacter pylori in DNA preparations obtained from feces or tissues of the stomach or duodenum of infected individual.The Real Time PCR reaction is a duplex-type. Bacterial DNA is detected in the FAM channel. The second channel (HEX) serves for detection of the internal control. Controls included into the test (recombinant DNAs) allow for monitoring the correctness of the Real Time PCR reaction.

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