Opakowania handlowe

AmpliTest Enterobius vermicularis

(Real Time PCR)


Human pinworm (Enterobius vermicularis ) is a white nematode parasite. It reaches size of approx. 1 cm (females) or approx. 3 mm (males). Human pinworm parasitizes only in the human body, in the large intestine, the appendix and the terminal section of the small intestine. It feeds on substances present in the intestinal contents. Females live about 4 weeks, and males die immediately after breeding. The female lays 8000-12000 eggs containing parasite larvae. Within 4-8 hours, the larvae achieve invasive ability. Pinworm causes a disease called enterobiasis. The course of enterobiasis depends on the number of parasites and host’s immunity. Sick children do not sleep well due to severe itching in the anal area. Enterobiasis causes also abdominal pain, defecation disorder, nausea, dermatitis, rash. The disease can lead to serious retardations in physical and mental development.

AmpliTest Enterobius vermicularis (Real Time PCR) test is designed to detect DNA sequences specific for pinworm in DNA preparations obtained from feces of an infected individual. The Real Time PCR reaction is a duplex-type. Pinworm DNA is detected in the FAM channel. The second channel (HEX) serves for detection of the internal control. Controls included into the test (recombinant DNAs) allow for monitoring the correctness of the Real Time PCR reaction.

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