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AmpliTest Escherichia coli

(Real Time PCR)


Escherichia coli is a gram-negative bacterium belonging to the Enterobacteriaceae family. More than 180 serotypes of this species have been described. E. coli is a symbiotic bacterium present in the intestine of humans and warm-blooded animals. It can also be found in soil and water, where it goes with the faeces. The presence of E. coli in water (so called Coli) is a common indicator of its pollution. The presence of Escherichia coli outside the digestive tract can lead to a variety of diseases, including peritonitis and urogenital tract infections. Particularly dangerous are the virulent strains of E. coli that produce toxins and/or are highly invasive. They can cause inflammation of the digestive system and diarrhea and cause septicemia. Young and immune-deficient organisms are especially susceptible to E. coli infection (colibacillosis). It is estimated that colibacillosis is responsible for up to half deaths of young animals.

AmpliTest Escherichia coli (Real Time PCR) test is designed to detect DNA sequences specific for Escherichia coli in DNA samples isolated from the tissues of an infected animal, faeces, urine or water. The Real Time PCR reaction is a duplex-type. Bacterial DNA is detected in the FAM channel. The second channel (HEX) serves for detection of the internal control. Controls included into the test (rekombinant DNAs) enable control of the correct course of the Real Time PCR reaction.

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