Diagnostic test AmpliTest Digestive System Parasites II (Real Time PCR) allows detection in a single reaction the DNA sequences specific to protozoans Dientamoeba fragilis and Blastocystis hominis in DNA preparations obtained from food, water and feces samples.
The Real Time PCR is a triplex-type. The FAM channel serves for detection of the DNA sequences specific for Blastocystis hominis whereas Dientamoeba fragilis-specific DNA sequences are detected in the Texas Red channel. The HEX channel is reserved for monitoring of the internal control amplification. Additionally, diagnostic panel contains negative and positive controls allowing to monitor the whole aspects of the Real Time PCR. Positive control was obtained using genetic engeeniring.