Diagnostic test AmpliTest Digestive System Parasites I (Real Time PCR) allows detection in a single reaction the DNA sequences specific for tapeworm Echinococcus granulosus and roundworms Ascaris lumbricoides hominis and Trichuris trichiura in DNA preparations obtained from feces samples.
The Real Time PCR is a fourplex-type. The FAM channel serves for detection of the DNA sequence specific for tapeworm Echinococcus granulosus, Cy5 serves for detection of the DNA sequence specific for roundworm Trichuris trichiura, whereas Ascaris lumbricoides hominis-specific DNA sequences are detected in the Texas Red channel. The HEX channel is reserved for monitoring of the internal control amplification. Additionally, diagnostic panel contains negative and positive controls allowing to monitor the whole aspects of the Real Time PCR. Positive control was obtained using genetic engeeniring.