AmpliTest Taenia solium
(Real Time PCR)
PAR01-100
Pork tapeworm (Taenia solium) is one of the species of tapeworm parasitizing vertebrates in the gastrointestinal tract, including humans. The length of its body can reach up to 4 meters. It is distinguished from a beef tapeworm by the presence of hooks and suction cups on its head enabling it to attach to the wall of the host’s small intestine. The body of a tapeworm consists of several hundred identical proglottids. The tapeworm development cycle is complex, requires two hosts: intermediate and definitive. In the case of the port tapeworm, the intermediate host is the domestic pig or the wild boar, the definitive host usually being a human. After the intermediate host has eaten tapeworm eggs excreted with faeces by the definitive host, the larva develops, which after being harboured encapsulates to take on the form of a cysticercus. After eating raw or undercooked/unfried pork, a cysticercus enters the human gastrointestinal tract. A tapeworm infection is called taeniasis and its course is generally mild. However, it can lead to the development of cysticercosis, which attacks the eyes or the nervous system. Clinical symptoms of tapeworm infection are non-specific and include: digestive disorders, abdominal pain, appetite disorders, headaches, nausea, disorders of the nervous system.
AmpliTest Taenia solium (Real Time PCR) test is designed to detect DNA sequences specific for tapeworm Taenia solium in DNA preparations obtained from feces samples of infected individual. The Real Time PCR reaction is a duplex-type. Tapeworm DNA is detected in the FAM channel. The second channel (HEX) serves for detection of the internal control. Controls included into the test (recombinant DNAs) allow for monitoring the correctness of the Real Time PCR reaction.